Enzymatic kinetics: determination of Michaelis constant


In the process of enzymatic hydrolysis of urea in an aqueous solution, carbon dioxide and ammonia are obtained. Their ions increase the conductivity of the solution. The speed of the process of hydrolysis of urea by the urease enzyme is measured by conductivity measurements at various concentrations and is determined by the Michaelis-Menton constant.

Training program

  • Michaelis-Menton mechanism
  • Reaction speed
  • Enzymatic kinetics
  • Bodenstein principle
  • Electrolytic conductivity

Technical specifications
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